Study on the capture of spores of Magnaporthe grisea by quantitative airborne spore traps

Rice blast is a major disease affecting rice production. It is a worldwide rice disease caused by the imperfect fungus Amon pear-shaped genus Pyrus. There have been reports of rice blast disease in 85 countries around the world. Loss. Its congruent spores are pear-shaped or inverted-stick-shaped. They are colorless and have no separation at the beginning. When mature, they have two septa. Individuals have 1 3 3 septa, bases have septa, and sometimes the septum is slightly retrenched. The temperature range for mycelial development of rice blast is 8-370C, and the optimum temperature is 26}28°C. The formation temperature of the constipation spores is 10350C. The optimum temperature is 2528°C. The minimum required time is 6}8h. The temperature rises and the production volume gradually decreases. When the temperature exceeds the climax, the birthing volume is very low. The formation of the spores was optimal at saturation humidity, decreased to 10% when the relative humidity was 90%, and could not be formed at a relative humidity of 80%. Therefore, many spores were formed at night. Quantitative airborne spore traps are important instruments for spore capture and play an important role in the research process.

The production, release, transmission, and infestation processes of the rice blast fungus spores are affected by many factors. Monitoring of air spumulation in the field conditions enables the timely mastering of the spatiotemporal dynamics of the rice blast fungus and the understanding of the initial inoculum size. The amount of re-infected bacteria can help to establish the best timing for prevention and control, and effectively control and manage the disease. The wood test will monitor the amount of airborne spores in the field through the quantitative airflow spore trapping device, and analyze the relationship between spews and meteorological factors.

The principle of using the quantitative air flow spore catcher is to increase the amount of air passing through the slides through the suction fan, so that the catching efficiency of the spores can be improved, and it can be applied when the initial bacteria amount is small in the early stage of the disease.) The installation height is 1.3m . Start up at 3:00 every morning and catch 2h. Two glass slides were coated with carbon tetrachloride, and the formula was 100 mL of carbon tetrachloride to dissolve 10 ‧ shishilin [+ }. Each slide was exchanged for the number of spheroids on slides to which the rice blast fungus was attracted.

Using the field microclimate observer next to the quantitative airflow spore trap, each large meteorological factor (including temperature, rainfall, relative humidity, wind speed, etc.) was monitored and recorded. Then analyze the relationship between meteorological factors and the amount of airborne spores detected by each large-scale rice blast fungus.

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